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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was located to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a crucial job in advertising and marketing cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are already explained. Two extra splice variants ended up noted but in-depth nucleotide sequence information and facts just isn't nevertheless out there.
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Blocking peptides are peptides that bind specifically towards the target antibody and block antibody binding. These peptide generally consists of the epitope recognized with the antibody.
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This information outlines more doable optimization of cellular and immunoassay parameters to be sure the very best effects are...
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This guideline outlines even more feasible optimization of mobile and immunoassay parameters to make certain the absolute best success are...
Antibodies sure to the blocking peptide now not bind for the epitope around the goal protein. By evaluating the staining within the blocked antibody versus the antibody on your own, one can see which staining is precise.
Target Information and facts Cyclin E belongs for the highly conserved cyclin household, whose users are characterized by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show unique expression and degradation patterns which add into the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and features like a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates with the G1-S stage boundary and it is degraded as cells development by S stage.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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